Analysis Note

ControlHeLa acid extract

Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).

Anti-monomethyl-Histone H3 (Lys27), Trial Size Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 27 (H3K27me1). This antibody has dot blot (DB) verified specificity and is validated in, ICC, WB, ChIP, ChIP-seq. Small pack size is ideal for screening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence R30;ARmeKSAR30; in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Epitope: Lys27

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit IgG in buffer containing 0.01 M phosphate buffer, pH 7.6, 0.125 M NaCl, 0.05% sodium azide and 30% glycerol.

Format: Purified

Quality

Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 µg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

Specificity

Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.

Broad species cross-reactivity expected, based on sequence homology.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

17 kDa