Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

This Anti-trimethyl Histone H3 (Lys9) Antibody, Alexa Fluor^® 647 Conjugate is validated for use in ICC for the detection of trimethyl Histone H3 (Lys9).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Linear peptide corresponding to Histone H3 trimethylated at Lys9.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Protein A purified

Purified rabbit conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1 % Sodium Azide.

Quality

Evalutated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys9) in HeLa cells.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed. Refer to Cat. No. 07-442 for observed molecular weight information.