Analysis Note

ControlHeLa whole cell lysate, U2OS cell lysate.

Application

Use Anti-dimethyl-Histone H3 (Lys9) Antibody (Rabbit Polyclonal Antibody) validated in DB, ICC, PIA, WB to detect dimethyl-Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9).

Dot Blot Analysis:
Specificity confirmed by an independent laboratory.

Peptide Inhibition:
Specificity was confirmed by the ability of 1 µM of the immunizing peptide to completely abolish detection of Histone H3 in western blot analysis of HeLa acid extracts (Figure A, Lane 4).

Immunocytochemistry:
Reported by an independent laboratory.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human Histone H3.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Routinely evaluated by Western Blot on HeLa acid extract and recombinant Histone H3 (cat. # 14-411).

Western Blot Analysis:
1:500 dilution of this lot detected dimethyl Histone H3 on 10 µg of HeLa acid extract but not on recombinant Histone H3.

Specificity

Expected to cross react with mouse, chicken, rat and fission yeast, but not budding yeast. Broad species cross-reactivity expected.

Storage and Stability

1 year at -20°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

17 kDa