Application

This Anti-monomethyl Histone H3 (Lys4) Antibody, Alexa Fluor^® 488 Conjugate is validated for use in ICC for the detection of monomethyl Histone H3 (Lys4).

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 07-436.

Immunogen

Linear peptide corresponding to Histone H3 monomethylated at Lys4.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Purified rabbit Alexa Fluor^® 488 conjugated antibody in buffer containing PBS with 15 mg/ml BSA and 0.1% Sodium Azide.

Quality

Evaluated by Immunocytochemisty Analysis in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected monomethyl Histone H3 (Lys4) in HeLa cells.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Target description

~17 kDa observed (Refer to Cat. No. 07-436)