Analysis Note

ControlHeLa cell extract

Application

Immunocytochemistry:
Reported by an independent laboratory.

Peptide Inhibition:
Specificity was confirmed by the ability of 1 µM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Anti-monomethyl-Histone H3 (Lys4) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H3 (Lys4) also known as H3K4me1, Histone H3 (mono methyl K4) & has been validated in ICC, IF, PIA, WB, ChIP-seq, DB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Epitope: Lys4

KLH-conjugated, synthetic peptide containing the sequence ART[meK]R30;, in which meK corresponds to monomethyl-lysine at residue 4 of human histone H3.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.

Quality

Routinely evaluated by Western Blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.

Specificity

Broad species to cross-reactivity is expected.

Recognizes monomethyl-histone H3 (Lys4), Mr 17kDa.

Storage and Stability

Stable for 1 year at 2 to 8°C from date of receipt.

Target description

17 kDa