Analysis Note

ControlHuman outer cornified layer whole cell lysate

Application

Research Sub CategoryGeneral Post-translation Modification

Research CategoryInflammation & Immunology

Detect Citrulline with Anti-Citrulline Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.

Immunogen

Linear peptide containing Citrulline.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

ImmunoAffinity Purified

25mM Tris/Tris-HCl, pH 7.4, 137mM NaCl,2.7mM KCl, 0.02% sodium azide before the addition of glycerol to 30%

Quality

routinely evaluated by immunoblot in whole cell lysates from tape-stripped human outer cornified layers

Specificity

Multiple deiminated keratins in human cornified skin. Thought to react preferentially with citrulline residues in glycine rich contexts.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

43-65 kDa