Application

Detect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.

Research CategoryNeuroscience

Research Sub CategoryOxidative Stress

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS

Immunogen

Synthetic peptide corresponding to amino acids 106-118 of human eNOS/NOS III (Ser114) (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with pig and bovine eNOS/NOS III.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Immunoaffinity Chromatography

0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, and 0.05% sodium azide before the addition of glycerol to 30%

Quality

routinely evaluated by immunoblot in fetal bovine heart endothelial cell lysate

Specificity

eNOS/NOS III phosphorylated on serine 114

Storage and Stability

1 year at -20°C

Target description

132 kDa