Analysis Note

Control3T3/A31 lysate.

Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 µL of 2-mercapto-ethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Application

Immunocytochemistry:
A previous lot of this antibody showed positive immunostaining for PAR-3 in MDCK cells fixed with 3% paraformaldehyde followed by permeabilization with 0.1% Triton X-100 has been shown by an independent laboratory.

Anti-Partitioning-defective 3 Antibody is an antibody against Partitioning-defective 3 and has been published in over 20 citations for use in IC and WB.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PAR3 is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR3 interacts with PRCKI. PAR3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR3 interacts with F11R/JAM1. Antibodies against PAR3 are present in sera from patients with cutaneous T cell lymphomas.

Immunogen

GST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABS174

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A chromatography

Format: Purified

Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.

Quality

Evaluated by western blot on RIPA lysates from 3T3/A31, A431, and HeLa cells.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected PAR-3 isoforms in RIPA lysates from 3T3/A31, A431, and HeLa cells.

Specificity

Recognizes all 3 slice isoforms of PAR-3, MW ~180, 150, 100 kDa.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

~180, 150 & 100 kDa