Analysis Note

ControlHeLa cells, extracts from 3T3/NIH or HepG2 cells, human brain tissue

Application

Research CategorySignaling

Research Sub CategoryGrowth Factors & Receptors

Detect PDGFRα using this Anti-PDGFRα Antibody validated for use in IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.

Immunogen

peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF Receptor α

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A Purified immunoglobulin in 0.14 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

ImmunoAffinity Purified

Quality

routinely evaluated by immunoblot on 3T3/NIH cell lysate

Specificity

Recognizes PDGF Receptor alpha.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

150/170 kDa (doublet)