Analysis Note

ControlIFN-gamma then IFN-alpha treated HeLa cell lysate

Application

Western Blotting Analysis:
A a representative lot of this antibody detected phospho-STAT2 (Tyr689) in 10 µg of IFN alpha/gamma treated HeLa cell lysate.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated phospho-STAT2 (Tyr689) from IFN alpha treated HeLa cell lysate.

Detect phospho-STAT2 (Tyr689) also known as signal transducer & activator of transcription 2 with Anti-phospho-STAT2 (Tyr689) Antibody (rabbit polyclonal antibody), that has been demonstrated to work in WB.

General description

The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia. STAT2 (113 kDa), originally purified from the nuclei of a-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta. Knockout mice with a targeted disruption of STAT2 exhibit greater susceptibility to viral infection and altered responses to Type I interferons. Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Specificity

Recognizes STAT2 phosphorylated at tyrosine 689.

Target description

130 kDa