Analysis Note

ControlPhosphorylated and non-phosphorylated NF-kB peptides

Application

Research Sub CategoryTranscription Factors

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot blocked phospho-NF-kB (p100) (Ser707) in non-starved HEK293 cell lysates.

Research CategoryEpigenetics & Nuclear Function

Use Anti-phospho-NF-kB (p100) (Ser707) Antibody (Rabbit Polyclonal Antibody) validated in WB, PIA, DB to detect phospho-NF-kB (p100) (Ser707) also known as Nuclear factor NF-kappa-B p100 subunit or H2TF1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NF-kappa-B (NF-kB) is a family of ubiquitous transcription factors, which includes RelA, RelB, NF-kB1 and NF-kB2. The NF-kB family regulates multiple cellular processes, including cell growth, differentiation, inflammation, immunity, and apoptosis. In general, inactive forms of NF-kB proteins, bound by IkappaB inhibitory proteins, are present in the cytoplasm, whereas activated forms translocate to the nucleus where they exert transcriptional activity. The p100 subunit is an inactive precursor of NF-kB2, and is proteolyzed to generate the functional p52 protein and enable the formation of an active NF-kB2/p52 dimeric complex. The processing of p100 to form p52 is induced by the NF-kappaB-inducing kinase (NIK), which recruits IkappaB kinase alpha to p100, resulting in phosphorylating of p100 at serines 99, 108, 115, 123, and 872, which is required for subsequent ubiquitination and processing of p100 by the beta-TrCP ubiquitin ligase. Nuclear p100 is phosphorylated at serine 707 by GSK3, which promotes its degradation by Fbxw7α.

Immunogen

Epitope: Phosphorylated Ser707

KLH-conjugated linear peptide corresponding to human NF-kB (p100) phosphorylated at Ser707.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Dot Blot Specificity Analysis: Phosphorylated and non-phosphorylated NF-kB peptides (see Table) were probed with Anti-phospho-NF-kB (p100) (Ser707) (1:500 dilution).

Specificity

This antibody recognizes NF-kB (p100) phosphorylated at Ser707.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed