Analysis Note

ControlUntreated and insulin treated HepG2 cell lysate

Application

Research CategorySignaling

Research Sub CategoryCell Cycle, DNA Replication & Repair

Anti-phospho-SRPK2 (Ser494) Antibody is an antibody against phospho-SRPK2 (Ser494) for use in WB, PIA.

Peptide Inhibition Analysis: 1 µg/mL from a representative lot detected SRPK2 in 10 µg of insulin treated HepG2 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Along with Serine/threonine protein kinase 2 (SRPK2), SRPK1 phosphorylates serine residues found in RS-domain-containing proteins, such as SFRS1 and SFRS2. These kinases also phosphorylate the same serine residues on the HBV core protein both in vitro and in vivo. The cytoplasmic proteins are critical for nuclear import of SR proteins in a phosphorylation-dependent manner. Additionally, they mediate the trafficking of splicing factors and play a role in spliceosome assembly and mitogenesis.

Immunogen

Epitope: Protein kinase domain

KLH-conjugated linear peptide corresponding to the protein kinase domain of SRPK2 phosphorylated at Ser494.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in untreated and insulin treated HepG2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SRPK2 in 10 µg HepG2 cell lysate.

Specificity

This antibody is specific for the protein kinase domain of SRPK2 phosphorylated at Ser494, independent of observed phosphorylation at Ser497.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed. Two isoforms at ~77 kDa and ~79 kDa may be observed in some cell lysates.