Analysis Note

ControlC2C12 cell lysate.

Application

Research Sub CategoryTranscription Factors

Chromatin Immunoprecipitation: A representative lot immunoprecipitated TAF3 using chromatin from transfected cells grown under conditions that favor expression from the MyoD:EGFP transgene promoter over expression from the endogenous MyoD promoter.

Research CategoryEpigenetics & Nuclear Function

Detect TAF3 using this Anti-TAF3 Antibody validated for use in Chromatin Immunoprecipitation (ChIP) and Immunoprecipitation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TAF3 is a subunit of TFIID that was initially identified through its interaction with a developmentally regulated transcription factor Bric a brac 1 (bab1), which is implicated in pattern formation and tissue morphogenesis in Drosophila. TFIID is a general or basal transcription factor that regulates initiation by allowing RNA polymerase II to recognize a core promotor. TAF3-TBPL2 complexes are found in myoblasts; however, upon differentiation into myotubes, they are replaced by TRF3-TAF3 complexes. Further research is still required to elucidate the roles and the development of the former to the latter complex.

Immunogen

GST-tagged recombinant protein corresponding to human TAF3.

Physical form

Rabbit polyclonal serum containing with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Immunoprecipitation in C2C12 cell lysate.

Immunoprecipitation Analysis: A 1:1,000 dilution of this antibody detected TAF3 in 500 µg of C2C12 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~180 kDa observed.