Application

Research CategorySignaling

Anti-eIF4G Antibody is an antibody against eIF4G for use in WB & IF.

Research Sub CategoryRNA Binding Protein (RBP)Insulin/Energy Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

eIF4F is a translation initiation factor containing three subunits: eIF4A, eIF4E, and eIF4G. This complex is a key component in promoting ribosomal binding to the mRNA. eIF4G interacts with both eIF4A and eIF4E though its carboxy and amino terminal domains, respectively. eIF4E binds to the cap structure of the mRNA, while the ATP-dependent RNA helicase eIF4A unwinds the secondary structure of the 5' UTR. eIF4G plays a critical role in coordinating the assembly of the eukaryotic initiation factors. It acts as a bridge to bind the cap-bound eIF4E, eIF4AI&II, the eIF3 complex, PABP1, and the kinase Mnk1 (Lejeune, F., et al., 2004) in a large eukaryotic initiation factor complex

Immunogen

Epitope: N-terminus

Synthetic peptide corresponding to N-terminal region of human eIF4G.

Physical form

Rabbit polyclonal serum with 0.05% NaN3.

Quality

Evaluated by western blot on HeLa cytoplasmic extract.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect eIF4G in HeLa cytoplasmic extract and lysates from 293T and S10 cells.

Specificity

Not tested on other species.

This antibody detects eIF4G at its N-terminal region.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

220 kDa