Analysis Note

ControlMouse kidney lysate.

Application

Research Sub CategoryTranscription Factors

Immunocytochemistry:
A 1:500 dilution of this antibody was used to detect FOXO4 in NIH/3T3 and A431 cells. Positive nuclear staining.

Research CategoryEpigenetics & Nuclear Function

Anti-FOXO4 Antibody is an antibody against FOXO4 for use in WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Forkhead box O4 (FOXO4 or AFX) is a member of the forkhead family of transcription factors, (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. FOXO4 has similar properties to FOXO1, FOXO3, and FOXO and is associated with acute leukemia. Upon phosphorylation, FOXO4 is translocated from the cytosol to the nucleus. FOXO4 has three phosphorylation sites at Thr32, Ser197, and Ser262. There are 2 pathways, Akt and reversible acetylation, involved in negative control of FOXO4, resulting in cell survival. FOXO4 represses smooth muscle cell (SMC) differentiation by interacting and suppressing myocardin. FOXO4 also down regulates HIF-1 alpha protein levels which prevent hypoxia response.

Immunogen

Synthetic peptide corresponding to the N-terminal region of FOXO4.

Epitope: N-terminus

Linkage

Replaces: MABE539; MABD181

Physical form

Affinity purified

Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Quality

Western Blot Analysis:
1:500 dilution of this antibody was used to detect FOXO4 in mouse kidney lysate.

Specificity

This antibody recognizes FOXO4 at the N-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10µL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Target description

~65 kDa