Analysis Note

ControlInsulin untreated and treated mouse embryonic fibroblast cell lysates

Application

Anti-phospho-Grb10 (Ser501/Ser503) Antibody detects level of phospho-Grb10 (Ser501/Ser503) & has been published & validated for use in WB.

Research Sub CategoryGlucose/Glycogen Metabolism

Research CategoryMetabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Growth factor receptor-bound protein 10 (Grb10) belongs to a small family of structurally related multi-domain adaptor proteins, which are known to be involved with a diverse array of functions, including cellular growth, metabolism, apoptosis, and cell migration. Grb10 is widely, but not uniformly, expressed in mammalian tissues, and there are various known isoforms of Grb10. It is shown to bind to and positively or negatively mediate signals from a number of activated receptor tyrosine kinases, including the insulin (INSR) and the insulin-like growth factor (IGF1R and IGF2R) receptors, in addition to a variety of growth factor receptors and intracellular molecules, in a context-dependent manner. In one study, mTORC1-mediated Grb10 phosphorylation is demonstrated to lead to feedback inhibition of the phosphatidylinositol-3-kinase (PI3K) and the extracellular signal-regulated, mitogen-activated protein kinase (ERK-MAPK) pathways in HEK 293T cells, in vivo. However, many aspects of the Grb10 adapter function remain unclear. Further research is still required to elucidate and validate the interactions and effects of GRB10 with various cellular targets.

Immunogen

Epitope: Phosphorylated Ser501/Ser503.

KLH-conjugated linear peptide corresponding to human Grb10 phosphorylated at Ser501/Ser503.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in insulin untreated and treated mouse embryonic fibroblast cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected Grb10 in 10 µg of insulin untreated and treated mouse embryonic fibroblast cell lysates.

Specificity

This antibody recognizes Grb10 phosphorylated at Ser501/Ser503.

Other homologies: Rat (91% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~66 kDa observed. Uncharacterized bands detected in some lysates. UniProt describes 4 isoforms produced by alternative splicing: Isoform 1 at 62.026 kDa, Isoform 2 at 60.848 kDa, Isoform 3 at 67.231 kDa, and Isoform 4 at 66.487 kDa