Analysis Note

ControlHuman brain lysate

Application

Research CategorySignaling

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Detect Calcineurin Aβ using this Anti-Calcineurin Aβ Antibody validated for use in WB, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Calcineurin is a serine/threonine protein phosphatase involved in the mediation of signal transduction in a wide variety of cellular responses. Calcineurin has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin′s multifunctional capabilities in the regulation of calcium signaling. The Aα form of calcineurin is a major catalytic subunit gene isoform of calcineurin in most tissues that allows differentiation of Aα from the Aβ and Aγ isoforms.

Immunogen

KLH-conjugagted synthetic peptide corresponding to Calcineurin Aβ.

Linkage

Replaces: AB1697

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 40% gylerol. Contains no preservative.

Quality

Evaluated by Western Blot in human brain lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calcineurin Aβ on 10 µg of human brain lysate.

Specificity

Recognizes the Aβ (polyproline) form of calcineurin, a less abundant gene isoform of calcineurin in most tissues. Allows differentiation of Aβ from the A α and A-γ isoforms. Reacts with calcineurin of all mammalian species.

Storage and Stability

Store at -20°C for up to 12 months in undiluted aliquots; at this temperature the solution will
remain unfrozen. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

Approx. 58 kDa