Analysis Note

ControlRat Brain Lysate

Application

This Anti-Cdc42 Antibody is validated for use in WB for the detection of Cdc42.

Research CategorySignaling

Research Sub CategoryMAP Kinases

Western Blot Analysis: Rat brain with previous lots of rabbit anti-Cdc42 (cat. # 07-1466) and rabbit anti-Rac1 (cat. # 07-14664).
The molecular weights of Rac1 and Cdc42 are ~21 kDa.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

cdc42 is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal Actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of cdc42 stimulates filopodia formation, whereas Rac results in the formation of lamellipodia, and Rho stimulates formation of actin stress fibers. Effectors of the cdc42/Rac pathway include NADPH Oxidase (Phox) and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of cdc42.

Immunogen

Synthetic peptide corresponding to amino acids 130-141 of human Cdc42
(AKNKQKPITPET-C).

Linkage

Replaces: AB4201

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative.

Quality

Routinely evaluated by Western Blot on rat brain lysates.

Western Blot Analysis: 1:500-1:2000 (0.5-2µg/mL) dilution of rat brain lysate with a previous lot of rabbit anti-Cdc42.

Specificity

Recognizes Cdc42, does not cross react with Rac1. Cdc42 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Cdc42 induces the formation of filopodia. Cdc42 can activate Rac (consequently, filopodia are intimately associated with lamellipodia.

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Target description

21 kDa