Analysis Note

ControlPervandate treated and untreated Huvec cell lysate

Application

Research CategorySignaling

Research Sub CategoryG-proteins

Anti-phospho-Paxillin (Ser126) Antibody is an antibody against phospho-Paxillin (Ser126) for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells.

Immunogen

KLH-conjugagted synthetic peptide corresponding to human Paxillin at phosphorylated Ser126.

Epitope: Ser126

Linkage

Replaces: AB3793

Physical form

Affinity purified

PBS (no Ca2+, no Mg2+), pH 7.3, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.

Quality

Evaluated by Western Blot in pervandate treated and untreated Huvec cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Paxillin on 10 µg of Pervandate treated and untreated Huvec cell lysate.

Specificity

This antibody recognizes Paxillin phosphorylated at Ser126.

Storage and Stability

Maintain at -20°C for up to one year or 2-8°C for short term use from date of receipt. Avoid repeated freeze/thaw cycles.

Target description

68 kDa