Analysis Note

ControlHuman brain lysate

Application

Research CategorySignaling

This Anti-Tie-2 Antibody, N-terminus is validated for use in WB for the detection of Tie-2.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tie-2 is a member of the tyrosine protein kinase family, is a transmembrane receptor for angiopoietin 1, and may represent one of the earliest markers for cell lineage within mammalian endothelial cells. Its expression is almost only found in human, mouse and rat endothelial cells and angioblasts. Most likely, Tie-2 regulates the differentiation, proper dispersal, and proliferation of these cells during angiogenesis. Mutations in TEK are a cause of VMCM or dominantly inherited venous malformations which are characterized by serpiginious, dilated veins. Tie-2 can be found in placenta and lung tissue. Lower levels of the protein can also be found in kidney, umbilical vein endothelial cells, and brain.

Immunogen

18 amino acid peptide sequence within the extracellular, N-terminus of human Tie-2

Linkage

Replaces: AB3126

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Affinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5, containing 0.2% BSA and 0.05% sodium azide.

Quality

Evaluated by Western Blot in human brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected Tie-2 in 10 µg of human brain lysate.

Specificity

Recognizes human Tunica internal endothelial cell kinase (TIE-2), also described as Angiopoietin-1 receptor, p140 TEK, or tyrosine protein kinase receptor. The peptide sequence used as immunogen is 100% conserved in human, rat, mouse, and 94% in bovine.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Target description

Approx. 126 kDa