Analysis Note

ControlPC3 cell lysate

Application

Research CategorySignaling

Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody detects level of SGLT-1 (Sodium Glucose Co-transporter-1) & has been published & validated for use in WB, IH(P), ELISA.

Research Sub CategoryInsulin/Energy Signaling

Immunohistochemisty (paraffin): Anti-SGLT-1 staining on human kidney, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1 µg/mL, using IHC-Select™ detection with HRP-DAB. Immunoreactivity is detected in the brush boarder of proximal convoluted tubule.

ELISA: 1:10,000 - 1:50,000 dilution of a previous lot, using 50-100 ng of control peptide/well.

Optimal working dilutions must be determined by end user.

Western Blot: A previous lot of this antibody was used on multiple lysates. See datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The kidneys play a major role in the regulation of blood glucose. Although kidney nephrons indiscriminatly filter about 180g of glucose per day from the blood, most is reabsorbed actively in the proximal convoluted tubule by a Na+/Gl co-transport (ratio 2:1) mechanism (SGLT). Typically, glucose is first reabsorbed by these transporters, which reside within the brush-border epithelia of the PCT cells. The glucose is then transported out of the cell across the basolateral membranes by a facilitated diffusion sugar transporter (GLUTs). At least three members of SGLT′s (SGLT1-3) have been cloned and characterized from various species. SGLT1-3 genes code for protein of approximately 659-672 residues, with both N and C termini predicted to be extracellular. There is approximately 60-70% homology between the 3 forms. SGLT1 does not discriminate a-MDG, glucose and galactose, while SGLT2 and 3 do not transport D-galactose efficiently. SGLT1 is an integral membrane protein expressed in proximal convoluted tubule Sq1 segments of the kidney (outer renal medulla) and in the intestine.

Immunogen

Synthetic peptide corresponding to amino acids 402-420 in the cytoplasmic region of SGLT-1 of mouse/rabbit small intestine.

Linkage

Replaces: AB1352

Physical form

Rabbit polyclonal IgG antiserum in buffer containing 0.1% sodium azide.

Quality

Routinely evaluated by Western Blot on PC3 lysates.

Western Blot Analysis:
1:500-1:2,000 dilution of this lot detected SGLT-1 on 10 µg of PC3 lysates.

Specificity

Recognizes SGLT-1.

Storage and Stability

Stable for 1 year at -20°C from date of receipt in undiluted aliquots.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Recognizes a band of approximately 70-77 kDa in Western blot.