Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GLUT-1 in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-1401) is shown to be suitable also for ELISA, Western blotting, immunocytochemistry, and immunohistochemistry applications.

Research CategorySignaling

Research Sub CategoryDevelopmental Signaling

This Anti-GLUT-1 Antibody, CT, Alexa Fluor^® 647 Conjugate is validated for use in Immunocytochemistry for the detection of GLUT-1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Solute carrier family 2, facilitated glucose transporter member 1 (UniProt P11166; also known as Glucose transporter type 1 erythrocyte/brain, GLUT-1, HepG2 glucose transporter, Human T-cell leukemia virus I and II receptor, Receptor for HTLV-1 and HTLV-2) is encoded by the SLC2A1 (also known as DYT9, DYT17, DYT18, EIG12, GLUT, GLUT1, HTLVR, GLUT1DS, PED) gene (Gene ID 6513) in human. Glucose transporters (GLUT-1 to GLUT-7) constitute a family of integral membrane glycoproteins that facilitate cellular glucose uptake. In addition to mediateing glucose uptake in adult tissues, GLUT-1 is the predominant glucose transporter in embryonic and fetal tissues. Two forms of GLUT-1 are found in human frontal cortex, the 55 kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.

Immunogen

Epitope: C-terminus

Synthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV).

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GLUT-1 in A431 cells.

Specificity

This antibody recognizes GLUT-1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~54 kDa observed