Application

Research CategorySignaling

Anti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP homolog 3 & has been validated in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.

Immunogen

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser423/425 of human Smad3.

Epitope: Phosphorylated Ser423/425

Physical form

Unpurified rabbit serum in buffer containing 0.05% sodium azide.

Quality

Evaluated by western blot on untreated and UV-treated AKR-2B cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect Smad3 in UV-treated AKR-2B cell lysate.

Specificity

Detects phosphorylated Smad3 (Ser423 and Ser425).

Storage and Stability

Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Target description

50 kDa