Application

This Anti-oxidized-CaM Kinase II (Met281/282) Antibody is validated for use in WB, IC for the detection of oxidized-CaM Kinase II (Met281/282).

An independent lab validated this antibody to work in immunocytochemistry in heart sections from mice treated with saline, AngII,or Iso (Erickson, J.R., 2008).

General description

The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

Immunogen

Epitope: Oxidized Met281/282

Synthetic peptide encompassing and including oxidized Met281/282.

Linkage

Replaces: 04-1079

Quality

Evaluated by western blot on untreated and H2O2-treated mouse heart cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect oxidized CaM Kinase II in mouse heart cell lysates.

Specificity

Expected to cross-react on Mouse and Rat based on 100% homology with immunogen sequence.

This antibody recognizes CaM Kinase II when oxidized on Met281/282.

Storage and Stability

Stable for 1 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Target description

60 kDa