Analysis Note

ControlHeLa cell lysate

Application

Anti-PP6R3 Antibody detects level of PP6R3 & has been published & validated for use in WB, IH(P).

Research CategorySignaling

Research Sub CategoryKinases & Phosphatases

Immunohistochemistry Analysis: 1:200 dilution of a representative lot detected PP6R3 in human kidney and human breast tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PP6R3 (protein Ser/Thr phosphatase 6 regulatory subunit 3), also known as SAPS3 is a regulatory subunit of protein phosphatase 6 which is important for cell survival and is associated with TOR signaling in yeast. PP6R3 has been implicated in the transition from G1 to the S phase of mitosis. The PP6R3 subunit is one of three human versions of SAPs which may also have a role to play in regulation of NF-kappaB.

Immunogen

Histidine-tagged-recombinant protein corresponding to human PP6R3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected PP6R3 on 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130-140 kDa observed. UniProt entry describes 6 isoforms produced by alternative splicing ranging from 88.9 to 98.9 kDa