Analysis Note

ControlModified and non modified peptides of acetylated and non-acetylated STAT5B protein

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Detect acetyl STAT5B (Lys701) using this Anti-acetyl STAT5B (Lys701) Antibody validated for use in DB & WB.

Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Ma, L., et al. (2010). Proc Natl Acad Sci U S A. 107(45):19314-19319.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

STAT5B (signal transducers and activators of transcription 5b) controls the expression of early response genes and takes part in cytokine signaling. Studies have linked this protein to the inhibition of NFkappaB-mediated signaling. Additional studies have shown an association of STAT5B with regulation of lactation and growth, performance, and control of adipocyte function. Constitutively active STAT5B expression is often observed in a large number of leukemia's and solid tumors.

Immunogen

KLH-conjugated linear peptide corresponding to human STAT5B acetylated at Lys701.

Epitope: Acetylated Lys701

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Dot Blot in modified and non modified peptides of acetylated and non-acetylated STAT5B protein.

Dot Blot Analysis: 1 µg/mL of this antibody detected STAT5B in modified and non modified peptides of acetylated and non-acetylated STAT5B protein.

Specificity

This antibody recognizes STAT5B acetylated at Lys701.

Other homologies: Rat and Mouse (91% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

90 kDa calculated