Analysis Note

ControlHuman brain tissue lysate

Application

Research Sub CategoryPI3K, Akt, & mTOR Signaling

Research CategorySignaling

Anti-PHLPP1 Antibody detects level of PHLPP1 & has been published & validated for use in WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PHLPP1 is the protein phosphatase responsible for dephosphorylation of the Akt (Ser473) site. Akt (Ser473) is the TORC2 (mTOR) phosphorylation activation site.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of PHLPP1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Quality

Evaluated by Western Blot in Human brain tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected PHLPP1 in Human brain tissue lysate.

Specificity

This antibody recognizes the C-terminal region of PHLPP1.

Storage and Stability

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Target description

134 kDa Observed at 110 kDa