Analysis Note

ControlA431 Cell Lysate

Application

Research CategorySignaling

Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta

Optimal working dilutions must be determined by the end user.

Research Sub CategoryCytoskeletal Signaling

This Anti-phospho-MARCKS (Ser152/156) Antibody is validated for use in WB, IH(P) for the detection of phospho-MARCKS (Ser152/156).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin. Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose).

Immunogen

Synthetic peptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.

Epitope: phosphoSer 152/156

Linkage

Replaces: AB5879

Physical form

Affinity purified

Purified rabbit polyclonal IgG in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.

Quality

Routinely evaluated by Western Blot on EGF treated A431 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated MARCKS on 10 µg of EGF treated A431 lysates.

Specificity

MARCKS, phosphoSer152/156.

Storage and Stability

Stable at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~80 kDa