Application

Use Anti-Notch1 Antibody, Intracellular (Rabbit Polyclonal Antibody) validated in ELISA, WB, IHC(P) to detect Notch1 also known as Neurogenic locus notch homolog protein 1 precursor.

ELISA: 1:20,000-1:100,000 dilution from a previous lot was used in a standard sandwich ELISA assay against the peptide immunogen.
Optimal working dilutions must be determined by the end user.

Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining IGF-1 With Epitope Retrieval: Lipoma.

General description

Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.

Immunogen

Synthetic peptide from human Notch 1.

Linkage

Replaces: 04-1046

Physical form

Rabbit polyclonal IgG serum in buffer containing in 0.02 M Potassium phosphate, 0.15 M NaCl with 0.01% sodium azide.

Quality

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected NOTCH1, intracellular (Cleaved form) on 10 µg of HeLa lysates.

Specificity

Notch 1, intracellular. No reactivity against human Notch 2.

Reactivity with other species has not been determined.

Target description

~80 kDa