Analysis Note

ControlJurkat Cell Lysate

Application

Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-20 µg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP6 Polyclonal Antibody:
Human Testis
PP6 staining of round spermatids in normal human
testis, tissue pretreated with citrate buffer, pH 6.0.
Antibody was diluted 1:100, using IHC-Select™
detection with HRP-DAB.

Research CategorySignaling

Detect PP6C using this Anti-PP6C Antibody validated for use in WB, IP, IH(P).

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression.

Immunogen

The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Ala-Val-Pro-Asp-Ser-Glu-Arg-Val-Ile- Pro-Pro-Arg-Thr-Thr-Thr-Pro-Tyr-COOH.

Linkage

Replaces: AB4091

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal IgG in buffer containing PBS with 0.08% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP6C on 10 µg of Jurkat lysates.

Specificity

Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kDa.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Recognizes the catalytic subunit of Protein Phosphatase 6 (PP6), MW ~33kDa