Analysis Note

ControlL6 Cell Lysate

Application

Research CategorySignaling

Detect Protein Phosphatase 2 C α/β using this Anti-Protein Phosphatase 2 C α/β Antibody validated for use in WB.

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PP-2 C α/β is heterodimer located in the mitochondrial matrix. Within the pyruvate dehydrogenase complex, PP-2 C α/β serves to catalyze the dephosphorylation and concomitant reactivation of the E1 component's alpha subunit. Mutations occurring in the PDP1 gene are the cause of a disease known as pyruvate dehydrogenase deficiency (PDP deficiency), which leads to neurological dysfunction from lactic acidosis.

Immunogen

Purified peptide conjugated to KLH.

Linkage

Replaces: AB4090

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein Purified

Purified immunoglobulin in phosphate buffered saline with 0.08% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in L6 cell lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-2 C ALPHA/BETA on 10 ug of L6 lysates.

Specificity

Recognizes the alpha/beta regulatory subunit isotype of protein phosphatase 2C (PP2C). PP 2C alpha/beta polyclonal antibody binds a 44-46 kDa protein.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Target description

Approx. 46 kDa