Anti-PP1beta (rabbit polyclonal)

Analysis Note

ControlHeLa Cell Lysate

Application

Research CategorySignaling

This Anti-PP1β Antibody is validated for use in WB, IP, IH(P) for the detection of PP1β.

Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-15μg/mL.

Immunohistochemistry(paraffin):
Optimal Staining of PP1beta Polyclonal Antibody: Human Testis PP1beta staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select™ detection with HRP-DAB.

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.

Immunogen

The immunogen used to the purified peptide conjugated to KLH corresponding to the C-terminus of PP1 beta catalytic subunit (amino acids 318-327).

Epitope: C-terminus

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB4083

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified

Format: Purified

Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Quality

Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-1beta on 10 µg of HeLa lysates.

Specificity

Recognizes Protein Phosphatase 1β. No cross reactivity with other recombinant PP1 isoforms.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~ 38kDa