Analysis Note

ControlHEK293 cell lysate

Application

Research CategorySignaling

This Anti-PP2A Antibody, Catalytic subunit is validated for use in WB, IH(P) for the detection of PP2A.

Immunohistochemisty (paraffin): Antibody was diluted to 1:100, using IHC-Select™ Detection with HRP-DAB. Immunoreactivity is seen as staining in normal human testis.

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PP2A is a type 2 protein Ser/Thr phosphatase characterized by high activity against the α subunit of Phosphorylase Kinase and low sensitivity to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. PP2A is a heterotrimer containing a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism.

Immunogen

Synthetic peptide corresponding to a.a. 298-309 of the human sequence.

Epitope: C-terminus

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1621

Physical form

Affinity purified

Purified rabbit polyclonal IgG in buffer containing PBS with no preservatives.

Quality

Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution is traditonally used to detected PP2A, Catalytic subunit in 10 µg of HEK293 lysates.

Specificity

Peptide is highly conserved in human, rat, bovine, mouse and chicken.

Recognizes protein phosphatase 2A, catalytic subunit.

Storage and Stability

Stable for 1 year at -80°C from date of receipt. Avoid repeated freeze/thaw.

Target description

60 kDa