Analysis Note

ControlL6 Cell Lysate, plasma membranes from rat cerebrum.

Application

Research CategorySignaling

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Optimal working dilutions must be determined by end user.

This Anti-Gβ5 Antibody is validated for use in WB for the detection of Gβ5.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Unique peptide sequence derived from the D1 blade of the Gβ5 protein.

Epitope: D1

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB4068

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified

Purified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G protein Gbeta5 on 10 µg of L6 lysates.

Specificity

Recognizes D1 area of Gβ5.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa