Analysis Note

ControlHek293 cell lysate

Application

Research CategoryApoptosis & Cancer

Western Blot (SNAP ID) Analysis: 2 µg/mL from a previous lot detected IGF2 mRNA-binding protein 3 on 10 µg of Hek293 cell lysate.

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP.

Research Sub CategoryGrowth Factors & Receptors

This Anti-IGF2 mRNA-binding protein 3 Antibody is validated for use in WB for the detection of IGF2 mRNA-binding protein 3.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5′ UTR of the insulin-like growth factor 2 leader 3 mRNA and may repress translation of insulin-like growth factor 2 during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq].

Immunogen

KLH-conjugated linear peptide corresponding to human IGF2 mRNA-binding protein 3 at and around the C-terminus.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit serum in PBS with 0.09% azide.

Quality

Evaluated by Western Blot in Hek293 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected IGF2 mRNA-binding protein 3 on 10 µg of Hek293 cell lysate.

Specificity

This antibody recognizes IGF2 mRNA-binding protein 3 at the C-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

64 kDa was observed. Predicted to cross-react with all the Isoforms of IGF2BP3.