Analysis Note

ControlU2OS cell lysate

Application

Research Sub CategoryRNA Binding Protein (RBP)

Research CategoryEpigenetics & Nuclear Function

Anti-Upf1 Antibody is a rabbit polyclonal antibody for detection of Upf1 also known as ATP-dependent helicase RENT1 ,Nonsense mRNA reducing factor 1, Up-frameshift suppressor 1 homolog & has been validated in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Upf1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. Upf1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.

Immunogen

KLH-conjugated synthetic linear peptide corresponding to Upf1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by western blot on U2OS cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected Upf1 in U2OS cell lysate.

Specificity

This antibody detects Upf1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~125 kDa