Analysis Note

ControlMouse Brain lysate.

Application

Use Anti-MeCP2 Antibody (rabbit polyclonal antibody) validated in WB to detect MeCP2 also known as MeCP-2 protein, mental retardation X-linked 16, mental retardation X-linked 79, methyl CpG binding protein 2.

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.

Quality

Evaluated by Western Blot on Mouse Brain lysates.

Specificity

Recognizes MeCP2, Mr 75 kDa. A protein at ~75 kDa was also observed in HeLa nuclear extract. This protein was competed by pre-incubation of the antibody with the immunogenic peptide, suggesting that some HeLa strains contain MeCP2 or a related protein, contrary to a previous report.

Predicted to cross-react with human based on the conservation of the immunogenic sequence.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~75 kDa observed