Analysis Note

ControlL6 cell lysate, rat brain tissue extract.

Application

Immunohistochemistry (Paraffin):
10 µg/mL of a previous lot detected MnSOD in paraffin embedded rat brain tissue sections.

Anti-Mn-SOD Antibody is an antibody against Mn-SOD for use in IH, IH(P) & WB.

General description

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.

Immunogen

Recombinant human MnSOD (manganous superoxide dismutase).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified rabbit polyclonal in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Quality

Evaluated by western blot on RIPA lysates of MCF-7, A431 or rat L6 RIPA cells.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected MnSOD in RIPA lysates of rat L6 RIPA cells. 1-4 µg/mL of a previous lot detected MnSOD in RIPA lysates of human MCF-7, A431 and rat L6 RIPA cells.

Specificity

Recognizes MnSOD, Mr 24 kDa.

Target description

~24 kDa