Analysis Note

ControlMCF7 cell lysate

Application

Research Sub CategoryHormones & Receptors

Research CategoryEpigenetics & Nuclear Function

Anti-Estrogen Receptor α Antibody is a Rabbit Polyclonal Antibody for detection of Estrogen Receptor α also known as Estrogen receptor-like 1, Estrogen-related receptor alpha & has been validated in EMSA, ICC, IHC, IP & WB.

Immunoprecipitation Analysis: 0.2 µg/µL from a representative lot detected Estrogen Receptor a in MCF7 cell lysate.

Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected Estrogen Receptor a in MCF7 cell lysate.

Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Estrogen Receptor a in human breast cancer and uterine cancer cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of rat Estrogen Receptor alpha.

Epitope: C-terminus.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in MCF7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Estrogen Receptor a in MCF7 cell lysate.

Specificity

This antibody recognizes the C-terminus of Estrogen Receptor alpha.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~58 kDa observed. UniProtKB/Swiss-Prot P06211 (ESR1_RAT) lists a molecular weight of 67 kDa A molecular weight between 52-58 kDA due to variant isoforms may be observed in some cell lysates (Al-Bader, M.D. (2006). Reprod Biol Endocrinol. 4:13.; Geisler, J.G. et al. (2002). Diabetes. 51(7):2158-2169.). Uncharacterized bands may be seen at a molecular weight of ~10 kDa, ~35 kDa, ~41 kDa, ~105 kDa, and ~220 kDa in some cell lysates.