Analysis Note

ControlTSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60µl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.

Application

Research Sub CategoryHistones

Chromatin Immunoprecipitation:
5-10 µL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.

Research CategoryEpigenetics & Nuclear Function

Use Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) has been published and validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Immunogen

KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.

Epitope: a.a. 2-19

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Whole antiserum containing 0.05% sodium azide.

Unpurified

Quality

Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.

Specificity

Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.

Other species not tested, but expected to cross-react since Histone H4 is well conserved.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

10 kDa