Analysis Note

ControlLexA-pRB transfected yeast cell extract

Application

Detect LexA with Anti-LexA Antibody, DNA-binding region (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.

General description

The anti-LexA Antibody allows detection of LexA protein and recombinant proteins fused to the LexA protein. This antibody can be used to detect expression of potential bait proteins constructed for use with the yeast two-hybrid system or other interaction trap systems. LexA adds about 22 to 25 kDa to your protein, depending on the number of extra amino acids.

Immunogen

His-tagged DNA binding domain of LexA protein corresponding to amino acid residues 1-202

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Quality

routinely evaluated by immunoblot on LexA-pRB transfected yeast lysate

Specificity

Recognizes LexA portion of bait fusion proteins used in the yeast two-hybrid system.

Target description

24 kDa