Application

Research Sub CategoryNuclear Receptors

This Anti-acetyl-Histone H3 Antibody, Alexa Fluor^® 647 Conjugate is validated for use in Immunocytochemistry for the detection of acetyl-Histone H3.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H3 in NIH/3T3 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. The chromatin fiber is composed on repetitive units, known as nucleosomes, which are comprised of an octamer of core histones (two each of H2A, H2B, H3, and H4), around which DNA are wrapped. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Immunogen

Synthetic peptide corresponding to human histone H3 N-terminal sequence with acetylated Lys9 and Lys14

Epitope: N-terminus

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified rabbit antibody conjugate in buffer containing PBS, 15 mg/mL BSA and 0.1% Sodium Azide.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H3 in A431 cells.

Specificity

Antibody recognizes histone H3 with acetylated Lys9 and/or Lys14.

Other homologies: Broad species cross-reactivity expected based on sequence similarity.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

17 kDa observed