Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Anti-phospho-H3 (Ser10), Alexa Fluor^® 647 Conjugate is a purified rabbit polyclonal antibody to detect Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 647 and validated for use in IC. The unconjugated version of this antibody is published and proven in ICC, IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 06-570.

Immunogen

Linear peptide corresponding to Histone H3 phosphorylated at Ser10.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Protein A purified

Purified rabbit conjugated antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed (Refer to Cat. No. 06-570)