Analysis Note

ControlRat Brain Microsomal Prep. lysate.

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

This Anti-Na+/K+ ATPase α-1 Antibody is validated for use in IC, IP, WB for the detection of Na+/K+ ATPase α-1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Na+/K+ ATPase A-1 is a human gene and functions to maintain Na+ and K+ across the cell membrane which is important for osmoregulation. It is a component of (Na+/K+) ATPase, a transporter that catalyzes the hydrolysis of ATP and transports sodium and potassium ions across the plasma membrane. It also partners with Src and other signaling proteins. Binding of ouabain and other cardiotonic steroids to the (Na+/K+) ATPase activates Src, resulting in the assembly and activation of multiple signaling cascades. Binding of ouabain also induces a positive inotropic effect. Diseases associated with ATPase are Thyrotoxic periodic paralysis and hyperthyroidism. (References: Wikipedia and http://www.bioportfolio.com/gene/476-ATP1A1.html)

Immunogen

Epitope: Cytoplasmic domain

GST-tagged ATPase.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl and 0.05% NaN3.

Format: Purified

Quality

Routinely evaluated by Immunoprecipitation on rat brain microsomal prep. lysate.

Specificity

Expected to cross-react with Rat-100%, Mouse-99%; human, pig, rabbit, monkey-96%.

Recognizes the α-1 subunit isoform of Na⁺/K⁺ ATPase.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

112 kDa