Analysis Note

ControlJurkat cell lysate

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Detect c-Jun, also known as transcription factor AP-1, with Anti-c-Jun rabbit polyclonal antiserum, Cat. No. 06-225, that has been demonstrated to work in Immunocytochemistry, Immunoprecipitation, and Western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced
signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Immunogen

Trp E fusion-protein containing the DNA binding domain of avian c-Jun

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-210

Physical form

Whole rabbit antiserum containing 0.05% sodium azide

Antiserum

Quality

routinely evaluated by immunoblot on RIPA lysates from human A431 cells or in Jurkat cell lysate

Specificity

c-Jun

Storage and Stability

2 years at -20°C

Target description

35-39 kDa