Analysis Note

ControlLambda phosphatase treated and untreated Murine RAW cell lysates

Application

Detect phospho-TAK1 (Ser412) using this Anti-phospho-TAK1 (Ser412) Antibody validated for use in WB.

General description

TAK1 MAPKKK is activated by TAB1 to transform growth factor-beta (TGF-beta) signaling. TAK1 can also associate with TAB1 to form a unique MAPKKK complex that is thought to activate AP-1 and the NFkappaB signaling pathways. TAK1 has been linked to both IL-1alpha and TNFalpha signaling and therefore may also be involved in the inflammatory response. Recent research has found that the TAK1-TAB1 complex may have anti-inflammatory effects by aiding stercurensin in regulating the NFκB-dependent inflammatory pathways.

Immunogen

Epitope: Phosphorylated Ser412

KLH-conjugated linear peptide corresponding to human TAK1 phosphorylated at Ser412.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in lambda phosphatase treated and untreated Murine RAW cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected TAK1 on 10 µg of lambda phosphatase treated and untreated Murine RAW cell lysates.

Specificity

This antibody recognizes TAK1 phosphorylated at Ser412.

Target description

~70 kDa observed