Analysis Note

ControlDaudi cells

Application

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Detect Cyclin A with Anti-Cyclin A Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP, WB, ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins which are expressed and then degraded in a concerted fashion to drive the stages of the cell cycle. Cyclins combine with cyclin dependent kinases (cdks) to form activated kinases that phosphorylate targets leading to cell cycle regulation. A breakdown in the regulation of this cycle can lead to out of control growth and contribute to tumor formation. Defects in many of the molecules that regulate the cell cycle have been implicated in cancer. Cyclin A is a member of the Cyclin A family that can bind and activate Cdc2 and Cdk2 and is involved in the control of mitosis and S phase. During S phase, cyclin A forms a complex with Cdk2, the pRB-related protein p107 and E2F.

Immunogen

peptide corresponding amino acid residues 417-432 (C-KHGVSLLNPPETL-N-L)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein G Chromatography

Lyophilized

Format: Purified

Quality

routinely evaluated by immunoblot on HeLa nuclear extract

Specificity

p58cyclin A; does not cross-react with cyclin B or cyclin D2, or 3

Storage and Stability

2 years at -20°C

Target description

58kDa