Analysis Note

ControlC2C12 cell lysates.

Application

Use Anti-SLBP Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect SLBP also known as Histone stem-loop-binding protein, HBP, Histone RNA hairpin-binding protein.

Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of NIH/3T3 cells using 06-1376 (Red). Actin filaments have been labeled with AlexaFluor™488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains cytoplasm and nucleus.

Research Sub CategoryRNA Metabolism & Binding ProteinsHistones

General description

The SLPB gene (stem-loop binding protein) encodes a protein that binds to the stem-loop structure of replication-dependent histone pre-mRNAs. Histone mRNA lack a polyadenylation signal. Consequently, the interaction of this protein with histone pre-mRNA contributes to efficient 3′ end processing by stabilizing the complex between the pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP). Expression of this protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human SLBP.

Physical form

Serum in buffer containing 0.05% sodium azide

Quality

Evaluated by Western Blotting in C2C12 cell lysates.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected SLBP in C2C12 cell lysates.

Specificity

Antibody recognizes SLBP.

Target description

38 kDa