Analysis Note

ControlHeLa nuclear extract

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected mRNA-capping enzyme in NIH/3T3, A431, and HeLa cells.

Use Anti-mRNA-capping enzyme Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect mRNA-capping enzyme also known as mRNA-capping enzyme, HCAP1, HCE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

mRNA capping enzyme is one of the first mRNA transcription cycle factors to be associated with the C-terminal domain (CTD) and functions by adding an inverted guanylyl group (also known as a R16;cap') to the 5' end. mRNA capping enzyme also has triphosphatase and guanylyltransferase activity. The CTD also serves to regulate the capping enzyme and has the ability to remove the Ser5 phosphate which is linked to the release of the capping enzyme from the capping complex. mRNA capping enzyme has a variety of other functions during transcription including; stabilization of polymerase II, activation of early elongation and reduction of re-initiation.

Immunogen

GST-tagged recombinant protein corresponding to human mRNA-capping enzyme.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1:5,000 dilution of this antibody detected mRNA-capping enzyme on 10 µg of HeLa nuclear extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~66 kDa observed