Analysis Note

ControlRAW264.7 cell lysate

Application

Western Blot Analysis: 1 µg/mL from a previous lot detected JAK2 in 10 µg of L6 cell lysate.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JAK2 from RAW264.7 cell lysate.

Research CategoryInflammation & ImmunologySignaling

Research Sub CategoryImmunological SignalingKinases & Phosphatases

This Anti-JAK2 Antibody is validated for use in WB, IP for the detection of JAK2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Janus family of tyrosine kinases (JAK) includes JAK1, JAK2, JAK3 and Tyk2. This family associates with a variety of cytokine receptors and is activated by ligand binding. Upon ligand binding to cytokine receptors, JAKs phosphorylate themselves and their associated receptors. This provides multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including STATs, Shc, insulin receptor substrates and FAK.

Immunogen

KLH-conjugated linear peptide corresponding to the protein kinase 1 domain of mouse JAK2.

Epitope: Protein kinase 1 domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected JAK2 in 10 µg of RAW264.7 cell lysate.

Specificity

This antibody recognizes the protein kinase 1 domain of JAK2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa